The FluoTime 250 from PicoQuant is a Fluorescence Lifetime Spectrometer that uses picosecond pulsed lasers diodes and LEDs covering a spectral range from 255 to 1550 nm. In its basic configuration, the spectrometer uses a motorized filter wheel for wavelength selection. An optional monochromator for the UV/VIS spectral range is available. The FluoTime 250 is very sensitive and can reliably detect the fluorescence decay of samples with concentrations down to 10 pMol (measured on a coumarin sample). It can be used to study various samples and perform several applications, including Fluorescence decays, Phosphorescence decays, Time-resolved anisotropy, Time-resolved emission spectra with an optional monochromator and Lifetime kinetics.