Fluorescence Limit of Detection

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One of the first questions users of traditional benchtop fluorimeters ask is, “Does a compact diode array spectrometer have enough sensitivity for my measurement?” Using fluorescein as a benchmark, we show how quickly our WP VIS and quick-fit cuvette holder can collect superior spectra with picomolar sensitivity. Here, we’ll explain how limit of detection (LOD) & limit of quantitation (LOQ) are defined and measured, using fluorescein spectra collected in our WP VIS fluorescence system.