Lasers versus LEDs for Bioinstrumentation

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A laser is a true point source that can be focused to a much brighter spot than a LED. This minimizes the real cost per watt of using a laser source, reduces optical complexity, maximizes signal to noise, and increases instrument speed.In any instrument based on the detection of fluorescence and/or light scatter, the key to successful operation is the ability to maximize signal-to-noise ratio. This, in turn, is accomplished by delivering the necessary amount of useable excitation light (that is, light that actually excites the fluorescent probe) into the required illumination sample region, while minimizing the amount of wasted light that is of the wrong wavelength or in the wrong spatial location.