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Pixel shift in fluorescence microscopy

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Pixel shift in fluorescence microscopy

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  • Author: Prashant Prabhat, Turan Erdogan
Multicolor imaging in fluorescence microscopy is typically performed by sequentially acquiring images of different colors. An overlay of these images is used to study the relative spatial distribution of various types of cellular components. However, in order to ensure that such a composite image is a true representation of the biological phenomena under investigation, it is important to understand imaging artifacts such as “pixel shift” error in multicolor fluorescence imaging.

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