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Resolution

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Resolution

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Resolution in fluorescence microscopy is defined as the shortest distance between two points on a specimen that can still be distinguished. This is primarily determined by two factors; microscope resolution, which is the smallest object the microscope can resolve, and camera resolution, which is the ability of the camera to detect what the microscope can resolve.The maximum resolution of the microscope is a function of the numerical aperture of the objective lens and the emission wavelength of the sample, whereas camera resolution is determined entirely by pixel size.

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