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Maximizing Microscope Field Of View

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Maximizing Microscope Field Of View

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Microscope field of view (FOV) is the maximum area visible when looking through the microscope eyepiece (eyepiece FOV) or scientific camera (camera FOV), usually quoted as a diameter measurement (Figure 1). Maximizing FOV is desirable for many applications because the increased throughput results in more data collected which gives a better statistical measurement for detecting subtle effects and also decreases time needed at the microscopeThe FOV of a microscope is ultimately limited by a number of factors, such as the objective lens, the tube-diameter of the microscope’s internal optical-system, the eyepieces, the scientific camera sensor size and the camera mounting adaptor It’s usually possible to find the maximum FOV of the microscope by referring to the field number (FN) displayed on the eyepieces and on some objective lenses.

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