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The Effect of Camera Cooling on Signal to Noise Ratio

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The Effect of Camera Cooling on Signal to Noise Ratio

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Signal to noise ratio describes the relationship between the detected signal originating from the sample and the uncertainty involved in the measurement of that signal on a per-pixel basis. It is essentially the ratio of the measured signal to the overall measured noise. Most low-light microscopy applications look to maximize signal and minimize noise. Noise, in this case, does not refer to background fluorescence or what may be referred to as ‘sample noise’, this is noise generated by the scientific camera. It is important to note that all scientific cameras generate noise, the three main sources of which are read noise, photon shot noise and dark current

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