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Understanding Laser Sources for 2- and 3-Photon Excitation

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Understanding Laser Sources for 2- and 3-Photon Excitation

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  • Author: Marco Arrigoni, Erin Dlugosz
2-photon and 3-photon excitation each have their own unique laser requirements in terms of pulse energy, wavelength, repetition rate, and pulse width. As such, the fundamental 2- photon and 3-photon experimental techniques cannot be addressed by the same light source. The goal of this paper is to explore these specific laser requirements in depth, highlighting how they enable each type of excitation and presenting corresponding laser developments from Coherent that streamline both 2-photon and 3-photon applications.

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