Factors Influencing OD600 Measurements
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Factors Influencing OD600 Measurements
The turbidity measurement of microbial cultures is a widely used method to determine the cell number of growing microorganisms in a culture. This method is performed by measuring the absorbance value of a liquid microbial culture in a photometer at 600 nm. The determination of the turbidity of a microbial sample should be carried out in the same photometer for each repetitive experiment to avoid device related result variations. Furthermore the OD600 value can vary when the parameters for microbial growth, such as nutrient availability in the medium, temperature, shaking speed in the incubator or glassware used, change. It is therefore important to produce constant parameters for cell growth and carefully check these for each repetitive experiment for reproducible, reliable results.
