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Tech Note: Limit of Detection for Fluorescence Spectroscopy

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Tech Note: Limit of Detection for Fluorescence Spectroscopy

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In this tech tip, we’ll explain how limit of detection (LOD) and limit of quantitation (LOQ) are defined and measured, walking you through the data analysis using our WP VIS fluorescence system as an example with fluorescein as the analyte. By using our own highly efficient volume phase holographic (VPH) gratings in a diffraction-limited transmissive design, our spectrometers maximize sensitivity and reduce stray light significantly as compared to other compact spectrometers of similar cost. In fact, we feel obliged to show you the data to convince you of its picomolar sensitivity – it’s within a factor of 10 of much more expensive benchtop systems.

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