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Peak Power in Non-Linear Microscopy: Unraveling the Rhetoric

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Peak Power in Non-Linear Microscopy: Unraveling the Rhetoric

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  • Author: Darry McCoy, Marco Arrigoni
Just about every non-linear microscopy application of general interest, inclusive of optogenetics and physiology for in vivo neuroscience can be addressed with femtosecond pulses in the range of 50-200 fs (from the laser source). Laser sources producing pulses below ~100 fs benefit from the use of precompensation in order to avoid loss of excitation due to peak power degradation. The need for precompensation is less stringent at wavelengths above 1 micron because the GDD of all optical materials strongly decreases at longer wavelengths. Independently on the wavelength used, increasing average power and/or peak power ultimately leads to linear (thermal) or non-linear damage respectively.

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