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RTN: How to Reduce Fluorescence in Raman Spectroscopy

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RTN: How to Reduce Fluorescence in Raman Spectroscopy

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  • Author: Matthew Berry
This Technical Note discusses techniques for dealing with fluorescence in Raman spectroscopy. It covers various hardware and software considerations for reducing fluorescence and improving the quality of Raman spectra, including describing the effects of the laser excitation wavelength, the diameter of the confocal pinhole, the groove density of the diffraction grating, background subtraction, and photobleaching of the sample. Raman scattering and fluorescence are fundamentally different phenomena.1 In Raman scattering, which is an inherently weak effect, the interaction between a molecule and an incident photon causes the promotion of that molecule to a very short-lived ‘virtual’ excited state, and when it relaxes via a radiative transition it is returned to a different vibrational energy level within the electronic ground state.

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