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Ratiometric Analysis with the SC-41 Plate Reader

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Ratiometric Analysis with the SC-41 Plate Reader

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  • Author: Angela Flack
The SC-41 Plate Reader, a fluorescence plate reader, enables high throughput measurements on the FS5 Spectrofluorometer via the automated measurements of microwell plates. Standard and custom microwell plates can be measured containing up to 384 wells. Both spectral and lifetime measurements can be acquired, a unique feature of the SC-41. This technical note demonstrates the use of the SC-41 Plate Reader for ratio analysis. The emission spectra can be measured from each well where samples contain varying ratios of two fluorophores.

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