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RTN: Two-Photon Fluorescence Microscopy with the RMS1000 Confocal Microscope

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RTN: Two-Photon Fluorescence Microscopy with the RMS1000 Confocal Microscope

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  • Author: Stuart Thomson, Marco Ferreira, Angela Flack
Two-photon microscopy is an imaging technique where two infrared photons are simultaneously absorbed to generate shorter wavelength fluorescence. Two-photon absorption is a non-linear process with the absorption probability scaling quadratically with excitation intensity. This results in the fluorescence being restricted to a much smaller focal volume than in one-photon fluorescence microscopy as the focal point is the only point in space with enough photon density for two-photon absorption to occur.

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