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Batch Processing and Global Analysis of Fluorescence Lifetimes

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Batch Processing and Global Analysis of Fluorescence Lifetimes

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  • Author: Georgios Arvanitakis
This Application Note outlined the successful application of Edinburgh Instruments' FAST software for batch processing and global analysis in studying the lifetimes of a three-fluorophore mixture. The batch analysis approach treats the lifetimes of each component in the mixture as independent parameters, while the global analysis approach considers them as linked (global parameters). Both methods offer valuable insights into the lifetimes and relative weight distribution of the individual components within a multi-fluorophore mixture. The choice between these methods should be based on the specific samples being studied and their intended applications.

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