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Temperature-Dependent Quantum Yield of Fluorescence from Plant Leaves

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Temperature-Dependent Quantum Yield of Fluorescence from Plant Leaves

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  • Author: M. Tesa, S. Thomson, A. Gakamsky
Fluorescence of plants has long been used as an indicator of their photosynthetic efficiency. The intensity and spectral characteristics of fluorescence offer information on the energy transfer processes occurring under illumination. In this application note we present temperature-dependent measurements of absolute fluorescence quantum yield of perennial leaves from 77 K to 300 K. Steady state and quantum yield data have been acquired with an FLS1000 Photoluminescence Spectrometer incorporating a Cryosphere accessory. The Edinburgh Instruments Cryosphere allows accurate characterisation of the changes in quantum yield with temperature, accounting for any changes in absorption that depend on temperature.

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