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Multiphoton Imaging of Mouse Intestine

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Multiphoton Imaging of Mouse Intestine

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  • Author: Stuart Thomson, Angela Flack
Two-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both imaging techniques utilise femtosecond pulsed infrared excitation light to generate shorter wavelength light to image the sample but operate via fundamentally different physical processes (Figure 1). In 2PEF, two infrared photons are simultaneously absorbed by a fluorophore promoting it to an excited state which then radiatively relaxes emitting shorter wavelength fluorescence. In contrast, SHG is not an absorption and emission process.

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