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Time-Resolved Fluorescence Techniques to FRET Based Immunoassay

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Time-Resolved Fluorescence Techniques to FRET Based Immunoassay

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In this application note, we discuss FRET Fluorescence Based Immunoassay after time-resolved techniques have been applied. Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of the optical excitation from an excited electronic level of one molecule, called the donor, to a resonant electronic level of another molecule, called the acceptor. If the electronic levels of donor (D) and acceptor (A) are in a strong resonance the optical excitation can be up to 10 nm. Efficiency of energy transfer exponentially decays as a sixth power of D-A separation distance.

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