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Nobel Prize Winners Eric Betzig’s Lattice Light Sheet Microscopy

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Nobel Prize Winners Eric Betzig’s Lattice Light Sheet Microscopy

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Since the invention of the microscope, we have learned a lot about biological processes. But the more details we find, the more we become aware of what is still to be discovered. By applying coherent light sources like lasers many new possibilities have emerged to improve resolution in microscopy and even to beat the limit of diffraction. Also Eric Betzig aimed at pushing these limits in microscopy. With PALM, for which he received the Nobel Prize, he developed a technique to resolve two neighboring fluorophores with a distance below the diffraction limit. His trick: He used a photoactivatable fluorescent protein for labeling, which can be stochastically activated with pulsed laser light of a certain wavelength. This way it is unlikely to excite two neighboring fluorophores at the same time. And after multiple acquisitions, an image reconstruction software calculates the final image from the time-series of fluorescent partial images.

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