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Trouble-free 2-Photon Microscopy

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Trouble-free 2-Photon Microscopy

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Two-photon (2P) absorption spectra of fluorescent dyes are broader than single-photon ones. Simultaneous 2P excitation of several dyes with a single wavelength (WL) is feasible and can be seen as an advantage. We used femtosecond (fs) pulsed fixed-WL infrared lasers with center WLs at 780 nm and 1040 nm, respectively, for Two-photon microscopy (2PM) in biologically relevant samples.

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