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Compact fixed wavelength femtosecond oscillators for multi-photon imaging

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Compact fixed wavelength femtosecond oscillators for multi-photon imaging

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  • Author: T. Hakulinen, J. Klein, Ruben Zadoyan, T. Baldacchini, T. Franke
In recent years two-photon microscopy with fixed-wavelength has raised increasing interest in life-sciences: Two-photon (2P) absorption spectra of common dyes are broader than single-photon ones. Therefore, excitation of several dyes simultaneously with a single IR laser wavelength is feasible and could be seen as an advantage in 2P microscopy. We used pulsed fixed-wavelength infrared lasers with center wavelength at 1040 nm, for two-photon microscopy in a variety of biologically relevant samples, among these a mouse brain sample, a mouse artery (within the animal, acute preparation), and a preparation of mouse bladder.

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