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Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator

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Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator

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  • Author: Carsten Tischbirek, Antje Birkner, Hongbo Jia, Arthur Konnerth
We introduce a two-photon imaging method with improved depth penetration for the recording of neuronal activity with single-cell resolution in the intact brain of living animals. This method relies on the use of the fluorometric Ca2+-sensitive dye Cal-590, which is effectively excited by infrared light (1,050 nm). By combining population Ca2+ imaging and electrical recordings in vivo, we demonstrate that neuronal activity can be monitored in all six layers of the mouse cortex. In combination with spectrally different Ca2+ indicators, Cal-590 can be used for the simultaneous imaging of neuronal activity in distinct neuronal populations.

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