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Raman in Life Sciences: Bacterial Phenotype & Inhibition Analysis

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Raman in Life Sciences: Bacterial Phenotype & Inhibition Analysis

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Raman scattering can provide similar compositional spectroscopic information to Fourier Transform Infrared (FTIR) absorption but without the complexities of using infrared sources, optics and detectors. And, unlike FTIR, Raman is particularly well-suited to aqueous samples making it a useful tool for in vitro, and potentially in vivo analytical applications in life sciences. Moreover, Raman’s wavelength flexibility and its compatibility with glass optics mean it can be combined with techniques such as fiber optics and microscopes for imaging and remote analysis. New technology from Ondax (now Coherent) has recently extended the analytical power of Raman spectroscopy from the traditional “chemical fingerprint” frequency region associated with vibrational resonances to the low frequency (THz) domain, providing unique information about phase (degree of crystallinity, polymorphism, etc.) effects, phonon scattering, etc.. In this whitepaper, we discuss why there is fast growing interest in Raman in life sciences, and examine some typical lasers used in this field.

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