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High Throughput Cancer Spheroid 3D Fluorescence Imaging and Morphology/Viability Analysis

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High Throughput Cancer Spheroid 3D Fluorescence Imaging and Morphology/Viability Analysis

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Cell-based drug discovery assays are traditionally done using two-dimensional (2D) cultured cells. While three-dimensional (3D) cultures provide a more biologically relevant environment, the data acquisition is slow. For example, capturing in-focus images from 3D samples requires stepping acquisition through multiple Z-planes for every XY position. Thus, scan times for three fluorophore experiments in 96 well plates can readily exceed 60 minutes. To overcome this time barrier, we developed the CYTOQUBE® Light-Sheet Microplate Cytometer. The CYTOQUBE uses light-sheet optics and intelligent software to speed up data acquisition in 3D.

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