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Direct Comparison of Fast Response and Slow Response Membrane Potential Dye using the FDSS6000

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Direct Comparison of Fast Response and Slow Response Membrane Potential Dye using the FDSS6000

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Ion transport proteins including channels, transporters, and pumps play central roles in cellular bioenergetics, excitability, and a multitude of other biological functions. Major approaches for ion channel drug screening include voltage clamp electrophysiology, radioactive flux or tracer assays, binding assays with whole cell or membrane preparations, and cellular fluorescent ion and membrane potential indicators. The use of cellular fluorescent probes is another important method that is used for drug discovery and development, especially for primary screening where high throughput assays are often required. Here we discuss cell-compatible fluorescent probes, functional assays, and FDSS instrumentation that are used to monitor real-time target activity and screen large chemical libraries for potent and selective modulators. High throughput screening (HTS) compatible, rapid kinetic assays are emphasized, in particular the use of slowresponse and fast-response voltage-sensitive membrane potential probes to assay ion channel activity in single cells and 96/384-well formats.

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