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Use of Ca2+ Oscillations in Primary Neuron Culture: A New HTS Model for neuronal Diseases

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Use of Ca2+ Oscillations in Primary Neuron Culture: A New HTS Model for neuronal Diseases

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Even though they have made their proof in the discovery of many broad spectrum antiepileptic drugs (AEDs), phenotypic screening strategies are limited by the use of in vivo models thus rendering the screening of large chemical libraries virtually impossible. Ca2+ oscillations play a physiologic role in the central nervous system and are thus involved in the study of epilepsy, pathological pain states, Parkinson disease and neurological disorders. When neurons are cultured in high density in low magnesium concentration, they enter a stable firing mode mimicking epilepsy. This model, widely used in academic research, had not been characterized before in 384 wells format for MTS. This study demonstrates that AEDs are active in this assay, thus confirming its relevance in MTS. Moreover, tests with ion channels modulators, GPCR or transporters show that this model can be used to study basic anticonvulsant MOAs

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