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FluxOR™ Differentially Identifies K+ Channel Antagonists Using FDSS6000

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FluxOR™ Differentially Identifies K+ Channel Antagonists Using FDSS6000

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Current assays for recording ion flux in cell based assays use No Wash Membrane Potential kits. These kits measure transmembrane voltage without regard to fluxing of specific ions. By contrast ion specific dyes like those measuring calcium channels (Fluo-4, Fura-2) are better probes as the specific ions fluxing across the membrane are identified. Invitrogen (IVGN) offers a fluorescent dye kit1) for measuring potassium channel activity using the surrogate ion Thallium. In this assay cells are loaded with the FluxOR dye. Cells are agonized through ligands or K+ in the presence of Thallium. Open K+ channels allow an influx of Thallium ions which binds to the dye; fluorescence increases. For K+ specific models IVGN offers BacMam vectors with one of a panel of K+ channels such as hERG.

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