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Detection and Modulation of Cardiomyocyte Calcium Oscillations Using the FDSS6000

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Detection and Modulation of Cardiomyocyte Calcium Oscillations Using the FDSS6000

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Current methodology to detect calcium oscillations in mouse fetal cardiomyocytes uses fluorescence microscopy to image cells plated on cover slips or Petri dishes. Using this model cells are exposed to putative cardiomodulators to investigate their pharmacological or toxicological properties. We report a methodology to transfer this model to high throughput screening using the FDSS6000. Wells show a baseline calcium oscillation (pulse) rate of either about 1/sec or appear quiescent. In both well types the agonist Isoproterenol increased pulse rate. In the latter wells we show the antagonist Propranolol blocks Isoproterenol agonism.

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