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Evaluation of a New Intracellular Calcium Fluorescent Reagent using the FDSS6000

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Evaluation of a New Intracellular Calcium Fluorescent Reagent using the FDSS6000

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G protein-coupled receptors (GPCRs) are trans-membrane spanning proteins that transduce an extracellular signal (ligand binding) into an intracellular event (G protein activation). The diversity of functions of GPCRs is related to the wide range of ligands recognized, ranging from small molecules (bioamines) to peptides and proteins (eg. chemokines). As a consequence, these receptors are involved in many physio-pathological conditions, which has led them to be an attractive class of targets for drug development. Institute de Recherches Servier has examined a new calcium fluorescent no wash assay kit from DiscoveRx Corp. for intracellular calcium release measurement using a Hamamatsu FDSS6000 imaging based plate reader. An aminergic and a peptidergic receptor were used as models to explore the potential of this new calcium probe, which was characterized in both agonist and antagonist modes, in different experi-mental designs corresponding to various HTS conditions.

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