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ß-arrestin Recruitment for GPCR Screening

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ß-arrestin Recruitment for GPCR Screening

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Over the years, it has been demonstrated that signaling through GPCR is not restricted to the type of G protein they are linked to. Downstream events can also be triggered by β-arrestin, a protein involved in signal regulation and receptor internalization. In order to detect βarrestin recruitment by GPCR activation, DiscoveRx has developed the PathHunter luminescent b-arrestin assay. The latest version of this assay is the PathHunter eXpress β-arrestin protocol which was tested on Hamamatsu FDSS instruments (7000 and 7000EX) and the new FDSS/µCELL. The FDSS/µCELL (a compact, cost effective and simple system for MTS and assay development) and FDSS7000EX are both equipped with Hamamatsu's new EM-CCD camera (Electron Multiplied) for fluorescence and luminescence assays and the FDSS7000 is equipped with our trademark photon counting camera. In this context, we compared FDSS7000 and FDSS7000EX in 1536 format using a proven and reliable assay such as PathHunter. The same experiment was conducted in 384 well format using the FDSS/µCELL. This study shows the comparison (assay window, EC50 and signal to noise ratio) between the two types of detection systems using the same assay.

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