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Incorporating Cell Viability Assays into High Throughput Screening Using the FDSS6000

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Incorporating Cell Viability Assays into High Throughput Screening Using the FDSS6000

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An antagonist assay measures calcium mobilization using a known agonist: receptor model following pre-incubation with a compound meant to block agonist binding. Signal inhibition indicates compound-mediated antagonism. However, inhibition may also result from compound: fluorescent dye quenching occurring inside cells1) or compound-mediated cellular toxicity. Cellular viability assays measure different stages of necrosis (accidental death) or apoptosis, (programmed cell death). Both necrosis and apoptosis proceed through welldefined temporal steps; numerous assays are available to measure various stages of death. For example, early in apoptosis mitochondrial membrane potential dissipates; JC-1 dye (M34152 IVGN, ex488 em529 and 590 nm) measures differences in mitochondrial membrane potential. Other assays for measuring apoptosis are commercially available.

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