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Effect of Plate Mixing, Fluid Addition Height and Speed on Reducing Addition Artifacts and Negative Control Drift Using the FDSS6000

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Effect of Plate Mixing, Fluid Addition Height and Speed on Reducing Addition Artifacts and Negative Control Drift Using the FDSS6000

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Two artifacts often present following fluidic addition to wells are) Sharp change in signal (either up or down) not associated with a biological response and) Drifting of the negative control well signal over the time course of the assay. Such artifacts are reduced using software algorithms including negative control correction (CeuticalSoft, Hudson, NY). However, the versatility of FDSS6000 hardware allows scientists to address addition artifacts, a more elegant solution than post assay data manipulation. In this study we compare the effects of cell plate shaking, pipettor fluid addition height, and pipettor fluid addition speed on reducing artifacts and improving assay response, using the FDSS6000

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