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Kinetic Luminescent Measurement of ROS (Reactive Oxygen Species) production with ABEL® Cell activation ROS kit with PHOLASIN® on the FDSS/µCELL platform

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Kinetic Luminescent Measurement of ROS (Reactive Oxygen Species) production with ABEL® Cell activation ROS kit with PHOLASIN® on the FDSS/µCELL platform

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Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in leucocytes, which is located in the cell plasma membrane and in the membrane of phagocytic vesicles inside the cell, transfers an electron of NADPH to outside of the cell or inside of the phagocytic vesicles across the membrane when it is activated. The electron reduces oxygen directly to generate superoxide, which leads to a number of reactive oxygen species (ROS) via a cascade of reactions. Such ROS production by leucocytes occurs when they are activated in the inflammation sites, and is a very important reaction in the immune system. The processes involved in the generation of ROS can be monitored using luminescence. Luminol and lucigenin have been used to measure ROS produced by the NADPH oxidase. However, their sensitivity is poor to require the isolation of ROS-producing cells from other cells in blood when the ROS production from leucocytes in blood is measured. The process of isolation of the correspondent cells changes the behavior of these cells making the relevance of these investigations less valuable for drug discovery.

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