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Comparison of Orthogonal Technologies to Confirm Agonist Hits Generated Using the FDSS6000

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Comparison of Orthogonal Technologies to Confirm Agonist Hits Generated Using the FDSS6000

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In this report we identify a subset of primary hits from a No Wash, fluorescence based agonist campaign using a recombinant cell line. We cherry picked the hits for retesting against a panel of assays to confirm activity CHO cells stably transfected with the coding sequence of a human receptor and Aequorin (Recombinant) or the parental cell line, stably transfected with Aequorin (Parental) were used in the study. The various assays, Aequorin (Euroscreen), IP-ONE (Cis-Bio), RT-CES (ACEA), Calcium No Wash (BD Biosciences), were used according to manufacturer's instructions.

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