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New Tools for Drug Discovery: Multiplexing the Monitoring of Ca2+ and cAMP Signaling in Primary Cells using the FDSS®/µCELL.

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New Tools for Drug Discovery: Multiplexing the Monitoring of Ca2+ and cAMP Signaling in Primary Cells using the FDSS®/µCELL.

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In drug discovery research cell-based assays are a widely accepted tool for high throughput screening of potential drug candidates. So far primary cells are not being used very frequently although they originate from the actual human tissue of interest, genuinely express the relevant drug targets, e.g. cell surface receptors at physiological levels, and carry the endogenous cofactors necessary for efficient and specific signal transduction. Thus, primary cells resemble the in-vivo situation and allow for a higher predictability of the situation in humans. Here we demonstrate the suitability of primary cells for signaling assays in compound screenings using Clonetics™ and Poietics™ primary sensors, i.e. primary and stem cells expressing luminescent biosensor proteins specific for Ca2+ and cAMP. These assay formats are used on the FDSS®/µCELL plate reader to generate pharmacologically relevant data for GPCR ligands.

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