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Anticancer drug screening in 2D/3D cultures using cell cycle marker Fucci

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Anticancer drug screening in 2D/3D cultures using cell cycle marker Fucci

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Fucci(CA)5 is a new variant of Fluorescent Ubiquitination-based Cell-Cycle Indicator that allows observation of cell cycle progression in live and fixed samples. The cell cycle phase is visualized by utilizing the ubiquitination domains of human Cdt1 (hCdt1(1/100)Cy(-)) fused with a red fluorescent protein (AzaleaB5), and human Geminin (hGem(1/110)) fused with a green fluorescent protein (h2-3), respectively.

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