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Cell Cycle Analysis Using Cell Cycle Indicator: Fucci

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Cell Cycle Analysis Using Cell Cycle Indicator: Fucci

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Fluorescent ubiquitination-based cell cycle indicator (Fucci) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells. Cdt1, which accumulates only in the G1 phase of the cell cycle, is fused with an orange fluorescent protein (monomeric Kusabira-Orange2:mKO2), and Geminin, which accumulates in the S/G2/M phases, is fused with a green fluorescent protein(monomeric Azami-Green1 :mAG1). The fluorescent intensity of each color is dependent on the level of expression of the Cdt1 and Geminin proteins. This application note introduces experimental examples of long-term time lapse analysis of Fucci-expressed HeLa cells observed by CellVoyager CV8000 and analyzed by CellPathfinder, clearly showing dose-dependent effect of Etoposide, an G2 arresting anti-cancer drug, on cell cycle population over time.

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