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Intracellular delivery of reagents with peptide or plasmids to primary cultured cells

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Intracellular delivery of reagents with peptide or plasmids to primary cultured cells

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The SU10 is able to directly deliver substances of interest into single cells’ nuclei or cytoplasm, using a “nano” pipette with a tip outer diameter of only a few tens of nanometers. Fish epithelial keratocytes have been used in many studies of cell motility because of their constant cell shape during their highspeed movement. However, these cells cannot be subcultured, which makes it difficult to introduce reagents and genes using conventional methods, such as electroporation. In this application note, we will demonstrate the use of the SU10 for introducing plasmids and a low membrane permeability fluorescent reagents into keratocytes and the use of the CSU confocal scanner unit for monitoring the cells.

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