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3D time lapse analysis of the growth of cultured embryonic stem cell colony and the expression of a pluripotency regulator protein

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3D time lapse analysis of the growth of cultured embryonic stem cell colony and the expression of a pluripotency regulator protein

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Embryonic stem cells (ES cells) have been a fundamental resource in gene engineering and regenerative medicine. It has been known that various transcription factors play crucial roles in the regulation of pluripotency of ES cells. Time lapse analysis in live cells has provided essential information regarding the temporal regulation of such pluripotency regulators. For example, a recent long-term single-cell tracking study in monolayer ES colonies revealed the less stringently implemented interactions of pluripotency regulators than assumed based on previous studies*1.

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