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Coherent Anti-Stokes Raman Scattering (CARS) Imaging Complements Other Multi-modal Imaging Techniques

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Coherent Anti-Stokes Raman Scattering (CARS) Imaging Complements Other Multi-modal Imaging Techniques

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  • Author: Philip G. Smith
Coherent Anti-Stokes Raman Scattering (CARS) Imaging has emerged in recent years as a powerful imaging technique, complementing traditional multiphoton imaging techniques such as multiphoton excited fluorescence (MPEF) and second harmonic generation (SHG) microscopy. The CARS method already described elsewhere1-8 enables label free imaging of biological samples exhibiting certain natural vibrational resonances. Specifically, CARS involves using two pulses of different wavelengths where the difference in frequency between the two pulses matches a molecular vibrational transition within the specific tissue of interest.

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