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Two-Photon Excited Fluorescence Microscopy

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Two-Photon Excited Fluorescence Microscopy

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Several laser-scanning microscopy techniques have been developed in the last twenty years based on non-linear optical phenomena.1 This has led to a variety of powerful imaging tools, such as two-photon excited fluorescence microscopy (TPM),2 second harmonic generation (SHG) microscopy,3 and coherent anti-Stokes Raman scattering (CARS) microscopy.4 The advantages these novel techniques present versus conventional microscopy methods have enabled great developments in biological imaging, especially in thick tissue and live animal studies.

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