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Fluorescence Imaging with Laser Illumination

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Fluorescence Imaging with Laser Illumination

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A typical fluorescence imaging or microscopy system is composed of three major components: an illumination source, a photo-activated fluorophore sample, and a detector. Any light incident on the sample within the sample’s excitation band will cause the fluorophore to emit light; typically the sample absorbs photons within its excitation band and emits photons at lower energy and longer wavelengths in its emission band. Two-photon fluorescence is another common fluorescence mechanism and may occur when a fluorophore is excited by a photon with a wavelength that is two times longer than a wavelength from its excitation band. Fluorescence that occurs this way requires two photons to reach the minimum energy needed to fluoresce because photons from this doubled-wavelength region have half of the energy.

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