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Light Sheet Microscopy

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Light Sheet Microscopy

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  • Author: Maura Francis, Cory Boone, Jaclyn Wycoff
Light sheet fluorescence microscopy (LSFM, also known as selective plane illumination microscopy (SPIM), is a medium-to-high-resolution fluorescence microscopy method that captures images at high speeds. It is well-suited for imaging three-dimensional biological samples at multiple depths, known as optical sectioning. A laser light sheet is focused into a two-dimensional plane, typically with a cylinder lens, to illuminate only a thin slice of the sample and excite fluorescence, reducing phototoxicity and damage to the sample

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