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Multiphoton Microscopy

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Multiphoton Microscopy

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  • Author: Maura Francis, Cory Boone, Jaclyn Wycoff
Multiphoton, or non-linear, microscopy, is ideal for capturing high-resolution three-dimensional (3D) images with reduced photobleaching and phototoxicity compared to traditional confocal microscopy techniques. This is the preferred method for analyzing thick samples of living tissue because of the technique’s advanced optical sectioning without out-of-focus absorption. Multiphoton microscopy is a subset of fluorescence microscopy that uses similar imaging techniques of conventional widefield fluorescence microscopy to differentiate structures in living cells or tissues with various fluorescent tags known as fluorophores. Laser wavelengths of sufficient energy will excite the fluorophores to emit light that generates an image.

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