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Confocal Microscopy

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Confocal Microscopy

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  • Author: Maura Francis, Cory Boone, Jaclyn Wycoff
Confocal microscopy is a type of fluorescence microscopy which uses a laser to excite fluorescence from fluorophores used to label different subsets of a specimen. Fluorescence microscopes are used for imaging cells and tissues that have been fluorescently tagged. What separates confocal microscopy from conventional epi-fluorescence microscopy is its increased resolution when imaging thick specimens, elimination of out-of-focus glare due to spatial filtering, and reduction of light-induced damage to the sample, known as phototoxicity.

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