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Microscopy and the Analysis of a Trichrome Stain

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Microscopy and the Analysis of a Trichrome Stain

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  • Author: Stephan Briggs
When imaging biological material, more often than not it is extremely difficult to differentiate between various organelles and tissues. Light scatters differently from each structure, but the change in contrast is so slight it becomes a strain to analyze the specimen. The first triple stain used to increase contrast and improve recognition dates back to 1880. One of the early methods of staining tissues for histology was developed by Claude Pierre Masson, and has since been coined the Masson trichrome stain.

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