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Optical Cage System Application: Differential Interference Contrast (DIC) Digital Microscope

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Optical Cage System Application: Differential Interference Contrast (DIC) Digital Microscope

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Differential interference contrast microscopy is a technique used to enhance contrast of object features that are otherwise difficult to observe using traditional brightfield microscopy. It does so by converting refractive index and/or surface slope or thickness variations—gradients in optical path length (OPL)—into intensity differences at the image plane. Slopes, valleys and surface discontinuities are imaged with improved contrast to reveal the profile of the surface. It is an effective method for accentuating differences in thickness or the refractive index of a sample, highlighting subtle features with vivid color gradients. Images produced with DIC give the appearance of a three-dimensional relief corresponding to the variation of OPL of the sample.

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