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Optical Microscopy Application: Brightfield Illumination

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Optical Microscopy Application: Brightfield Illumination

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  • Author: Stephan Briggs
Brightfield illumination, which yields dark objects on a bright background, is the simplest technique for optical microscopy. In brightfield illumination, the light source is positioned below the sample. Light then propagates through the sample, and is observed by the objective lens and sensor, which are positioned above the sample. The darker the sample, the denser the specimen, as denser samples absorb more light. The simplicity of brightfield illumination is the main reason this technique is so popular in optical microscopy.

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