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Optical Microscopy Application: Differential Interference Contrast

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Optical Microscopy Application: Differential Interference Contrast

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  • Author: Stephan Briggs
Differential Interference Contrast (DIC) is a polarization technique in optical microscopy that uses a polarizer, analyzer, and additional polarization optics such as a Nomarski or Wollaston prism. In simple DIC setups, the only required components are a polarizer and an analyzer. The polarizer is typically positioned below the specimen, and the analyzer above the objective lens. The polarization axis of each can be rotated to adjust contrast and throughput, but the two components typically act with respect to one another.

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