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Optical Microscopy Application: Fluorescence

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Optical Microscopy Application: Fluorescence

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  • Author: Stephan Briggs
Fluorescence microscopy is an optical microscopy technique that utilizes fluorescence, which is induced using fluorophores, as opposed to absorption, scatter, or reflection. A fluorophore is a type of fluorescent dye used to mark proteins, tissues, and cells with a fluorescent label for examination by fluorescence microscopy. A fluorophore works by absorbing energy of a specific wavelength region, commonly referred to as the excitation range, and re-emitting that energy in another specific wavelength region, commonly referred to as the emission range.

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